Fig. 4. Candidalysin does not affect intestinal permeability in mice fed ethanol.

C57BL/6 mice were fed an oral isocaloric (control) diet (1–2 technical replicates) or chronic-plus-binge ethanol diet (3–4 technical replicates) and gavaged with vehicle (PBS), wild type C. albicans (Wild type) or ECE1 deleted C. albicans (ece1Δ/Δ) with an amount of 10⁸ CFUs every third day. (A) Serum levels of FD4. Mice were gavage-fed FITC labeled dextran (200μl, 100mg/ml) 1 hour after binge on the last day and then sacrificed 4 hours later, and fluorescence was measured in the serum. (Control diet: PBS, n=6; Wild type, n=6; ece1Δ/Δ, n=5; Ethanol diet: PBS, n=5; Wild type, n=6; ece1Δ/Δ, n=6). (B) Serum levels of intestinal fatty-acid binding protein (IFABP) (Control diet: PBS, n=7; Wild type, n=5; ece1Δ/Δ, n=5; Ethanol diet: PBS, n=14; Wild type, n=13; ece1Δ/Δ, n=10). Results are expressed as mean ± s.e (A and B). P values were determined by two-way ANOVA with Tukey’s post-hoc test (A and B). ****P<0.0001.