Fig. 2.
Anatomical and histochemical analysis of GUS activity in PeHA1-pro::GUS-transgenic plants. Four-week-old seedlings of PeHA1-pro::GUS-transgenic plants were treated with 0 (−NaCl) or 200 mM NaCl (+NaCl) for 24 h. The wild-type (WT) controls were not treated with NaCl. Root, leaf, and stem samples were taken from control and salinized plants and used for anatomic analysis. (a) GUS staining. Scale bar=500 μm. (b) GUS activity was detectable in PeHA1-pro::GUS-transgenic plants regardless of the presence of salt stress, but was not detectable in WT plants. Each column corresponds to the mean of three independent replicates, and bars represent the SEM. **P<0.01. (This figure is available in color at JXB online.)