Fig. 8.

H⁺ fluxes and membrane potential in roots of wild-type (WT) tobacco and PeWRKY1-transgenic plants under salt stress. (a) H⁺ fluxes at the root tip. WT, vector control (VC), and transgenic lines (L-9 and L-12) were subjected to 0 (–NaCl) or 200 mM NaCl (+NaCl) for 24 h. The roots were then exposed to sodium orthovanadate (500 μM) for 30 min. Steady-state H⁺ fluxes were continuously recorded for 10 min at the apical zones (200 μm from the root tip) in the absence or presence of the inhibitor. (b) Membrane potential in control and salinized roots was continuously recorded for 10 min at the apical region (200 µm from the tip). Each column is the mean of 5–6 individual plants, and error bars represent the SE. Columns labeled with different letters denote a significant difference (P<0.05). (This figure is available in color at JXB online.)