iRhom2 increases in adipose tissues and promotes adipose inflammation in obese mice. (A) Western blotting for the expression of iRhom2, TNFR1/2, TNF-α, and phosphorylated NF-κB in iWAT harvested from standard chow diet and high-fat diet-fed C57BL/6N mice. (B) Western blotting of isolated adipocytes in iWAT of SCD and HFD-fed C57BL/6N mice. (C) qPCR expression changes in iRhom2, TNFR1/2, and TNF-α from iWAT or adipocytes in SCD and HFD-fed C57BL/6N mice; n = 6. (D) Western blotting of iWAT from db/db and corresponding mice. The data are expressed as mean ± SEM, *p < 0.05.