Figure 2.

iRhom2 deficiency exhibits decreased fat mass and insulin resistance. (A) Body weight of male C57BL/6N WT and iRhom2 KO mice during SCD and HFD feeding, n = 15. (B) Fat mass and (C) lean mass in male C57BL/6N WT and iRhom2 KO mice with SCD and HFD feeding, n = 15. (D) Representative photos of HFD-fed WT mice, iRhom2 KO mice, appearance of the liver, and Oil Red O stained hepatic sections (100× magnification). (E) Representative immunoblot bands of the levels of insulin resistance-associated signaling expression including phosphorylated IRS1^Y608, AKT, GSK3β, FOXO1, and PEPCK in iWAT and eWAT from HFD-fed WT and iRhom2 KO mice. (F) Glucose tolerance test and (G) insulin tolerance test of HFD or SCD-fed WT and iRhom2 KO mice, n = 15. The data are expressed as mean ± SEM. *p < 0.05 vs iRhom2 KO/HFD. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)