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. 2020 Feb 13;11:114. doi: 10.3389/fmicb.2020.00114

FIGURE 3.

FIGURE 3

Enzymatic activity of PE-H and different variants constructed by site directed mutagenesis. Substrates were (A) 4-nitrophenyl butyrate (pNPB), (B) bis(2-hydroxyethyl) terephthalate (BHET), (C) amorphous PET film, and (D) PET from a commercial single use bottle. Standard deviations of three individual reactions are shown as error bars. The respective substrate is depicted above each diagram. For pNPB and BHET, structural formulas are shown; for amorphous and commercial PET a cartoon representation is shown to illustrate the different arrangement of PET fibers.