Fig. 3.

MLL3 and SET1A decrease gene expression of ERα and its downstream target genes and cell-growth. The MCF7, MCF7/S0.5, or MCF7/TAMR-1 cells were transfected with siMLL3 or siSET1A for 48 h. a Total RNA obtained from the MCF7 cells were subjected to qRT-PCR analysis. Data presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001. b The whole cell lysates obtained from MCF7/S0.5 or MCF7/TAMR-1 cells were subjected to western blotting. c The number of viable cells were counted using a hemocytometer. Cell numbers were presented as the mean ± SEM from duplicate plates. One of three independent experiments with similar results are presented. ***P < 0.001. d Transfected cells were stained with propidium iodide and analyzed by flow cytometry for cell-cycle status