Fig. 4.

Inhibition of MLL3 and SET1A expression enhances sensitivity against fulvestrant-induced cell-growth inhibition in tamoxifen-resistant cells. a The MCF7/TAMR-1 cells were transfected with indicated siMLL3 or siSET1A for 48 h. Transfected cells were treated with the indicated concentration of fulvestrant (Fulv) for 24 h. The expression of ERα was determined by western blotting (top). Band intensities of ERα were quantified and normalized to that of α-tubulin. Data presented as mean ± SEM (n = 4). *P < 0.05 and **P < 0.01 (bottom). b The MCF7/TAMR-1 cells were transfected with indicated siMLL3 or siSET1A for 48 h. Transfected cells were treated with 5 nM fulvestrant for the indicated time periods and the number of viable cells was counted using a hemocytometer. Cell numbers were presented as the mean ± SEM from duplicate plates. One of three independent experiments with similar results are presented. ***P < 0.001