Fig. 5.

Effects of cholesterol manipulation. a Changes in cholesterol abundance in SH-SY5Y cells after a 12-h or 24-h treatment with 0 μM, 12.5 μM, or 25 μM 6-OHDA, in arbitrary units (a.u.). N = 4. b Effect of 10 nM to 5 μM simvastatin treatment on cell survival of SH-SY5Y cells treated with (6-OHDA) or without (control) 6-OHDA. N = 3. c Effect of 2.5 μM to 40 μM cholesterol treatment on cell survival of SH-SY5Y cells treated with (6-OHDA) or without (control) 6-OHDA. N = 4. d Intensity of β-III tubulin staining on SH-SY5Y cells treated with 0 μM, 2.5 μM, or 40 μM of cholesterol. Differences relative to the control. Representative images of SH-SY5Y cells treated with e 0 μM, f 2.5 μM, or g 40 μM cholesterol, stained with DAPI (blue) and β-III tubulin (red). N = 4. ^#p < 0.1 (trend); *p < 0.05; **p < 0.01; ***p < 0.001