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. 2019 Nov 1;42(1):19–38. doi: 10.1007/s11357-019-00103-0

Fig. 6.

Fig. 6

LD and mitochondrial localization of Erg13. A) mBAX expressionErg13-GFP localizes either to LDs or mitochondria. A heterogeneous expression level of Erg13-GFP is evident. (B) is a magnification of (A) and shows Erg13-GFP stained LDs. (C) is a magnification of (A) with a reduced exposure time. Erg13-GFP resides at mitochondria. (D) Erg13-GFP co-localizes with LD specific Nile red staining and LDs in the DIC image. (E) The GFP signal of Erg13 completely overlaps with DASPMI (a mitochondrial specific dye). (F) Immunoblot of purified LDs detecting the Erg13-GFP fusion using an anti-GFP antibody. 1: BY4741 ERG13::GFP::HIS3MX6 YEp51 (unstressed cells); 2: BY4741 ERG13::GFP::HIS3MX6 YEp51-mBAX (apoptotic cells); 3: BY4741 pUG35-vBAX (control cells). Erg13-GFP (83 kDa) shows a low abundance at isolated LDs obtained from unstressed cells and shows a high abundance at LDs isolated from apoptotic cells. The positive control vBAX-GFP (33 kDA) confirms the isolation of LDs. (G) Erg13-GFP shows a perfect co-localization with the DNA specific dye DAPI. In the images (A), (B), (C), (D) and (E) the BY4741 ERG13::GFP::HIS3MX6 YEp51-mBAX background is used (apoptotic cells), whereas in (G) the BY4741 ERG13::GFP::HIS3MX6 background (unstressed cells) is shown