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. 2020 Feb 13;11:17. doi: 10.3389/fphar.2020.00017

Figure 2.

Figure 2

miR-3568 mimic induced cell apoptosis and inhibited the expression of Survivin and Bcl-2 in simulated I/R-induced H9C2 cardiomyocytes. H9C2 cardiomyocytes were transfected with miR-3568 mimic or inhibitor and then simulated I/R for 24 h. (A) The expression of miR-3568 was measured by quantitative real-time PCR (n = 3). (B, C) The cell apoptosis was measured by flow cytometry (n = 3). The Survivin and Bcl-2 expressions were measured by quantitative real-time PCR (D; n = 3) and western blot (E, F; n = 3). Statistical analyses were carried out using one-way (A, B) or two-way (D, E) analysis of variance followed by Bonferroni post-test. ***P < 0.001 compared with control. ##P < 0.01, ###P < 0.001 compared with I/R + negative control (NC).