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. 2020 Feb 13;11:17. doi: 10.3389/fphar.2020.00017

Figure 6.

Figure 6

TRIM62 overexpression or AG490 treatment inhibits simulated I/R- and miR-3568 mimic-induced apoptosis and STAT3 activation in H9C2 cardiomyocytes. After H9C2 cardiomyocytes transduced with pLVX-Puro-TRIM62 and/or transfected with miR-3568 mimic and then simulated I/R, the cell apoptosis was measured by flow cytometry (A; n = 3) and the Survivin, Bcl-2, p-STAT3, and STAT3 expression was measured by western blot (B–D; n = 3). After H9C2 cardiomyocytes treated with AG490 (10 µM) and then simulated I/R for 24 h, the cell apoptosis was measured by flow cytometry (E; n = 3) and the Survivin, Bcl-2, p-STAT3, and STAT3 expression was measured by western blot (F–H; n = 3). Statistical analyses were carried out using one-way (A, C, E, H) or two-way (D, G) analysis of variance followed by Bonferroni post-test. ***P < 0.001 compared with control. ###P < 0.001 compared with I/R + Vector + NC. ΔΔΔP < 0.001 compared with I/R + TRIM62. ∇∇∇P < 0.001 compared with I/R + mimic.