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. 2020 Feb 13;11:17. doi: 10.3389/fphar.2020.00017

Figure 7.

Figure 7

miR-3568 inhibitor pre-treatment reduces cardiac I/R injury in vivo. miR-3568 inhibitor or its NC was injected into the left ventricular anterior wall of rats 24 h before I/R. (A) Representative photomicrographs of hematoxylin and eosin (HE), Masson, and terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL)-stained myocardium harvested 2 weeks post-I/R (n = 6). Quantization of fibrotic area (B; n = 6) and TUNEL-positive cells (C; n = 6). Cardiac functional parameters 2 weeks post-I/R were shown in (D) (n = 6). The expression of miR-3568, TRIM62, Survivin, Bcl-2, p-STAT3, and STAT3 in myocardium harvested 24 h post-I/R was measured by quantitative real-time PCR (E, F; n = 6) and western blotting (G; n = 6). (H) Schematic representation of the regulation of cell apoptosis by miR-3568/TRIM62/p-STAT3. Scale bar: 100 µm. Statistical analyses were carried out using one-way (B–E) or two-way (F, G) analysis of variance followed by Bonferroni post-test. **P < 0.01, ***P < 0.001 compared with control. #P < 0.05, ##P < 0.01, ###P < 0.001 compared with I/R + NC.