FIGURE 4.

Acute viability assays of ΔrarA double mutant strains upon exposed to MMS. Lack of RarA in cells impaired in end resection (A), processing of recombination intermediates (B), in RecA accessory proteins (C,D) or lack of RecD2 (D). Cells were grown to reach exponential phase (OD₅₆₀ = 0.4), exposed to different concentrations of MMS for 15 min prior to serial dilutions. Cells were counted as CFU after ON growth, and results are plotted dividing these CFUs by the CFU obtained in untreated cells. The results are the average of at least three independent experiments and standard errors of the mean are indicated.