FIGURE 5.

RecA protein accumulation upon SOS induction in different genetic backgrounds affecting RecA nucleoprotein dynamics. Exponentially grown wt (rec⁺), ΔrecX, ΔrecU, recF15, ΔrecO and ΔrarA cells (A) or wt, ΔrecX ΔrarA, ΔrecU ΔrarA and recF15 ΔrarA cells (B) were exposed to the indicated concentrations of MMC for 30 min. Then cells were collected, lysed and equivalent protein amounts subjected to 10% SDS-PAGE, followed by immunoblot transfer. The number of RecA molecules/CFU are derived from a standard curve of known RecA concentrations and are the average of at least three independent experiments and standard errors of the mean are indicated.