Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 19;11(2):136. doi: 10.1038/s41419-020-2329-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Rats were intratracheally instilled with a single dose of bleomycin (5.0 mg/kg) on day 0 and received microcystin-leucine arginine (LR) (20 μg/L) in drinking water starting on day 7 (LR7), 14 (LR14) or 28 (LR28). Rats were euthanized and samples were collected for analysis day 56 after receiving bleomycin intratracheal instillation. a Schematic representation of experimental design is shown. Data of two rats in Bleomycin group were excluded for further analysis due to animal dead. b Body weight gain after bleomycin treatment was calculated and shown as percentage increase over the baseline body weight. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01 determined by two-way ANOVA with Student–Newman–Keuls (S–N–K) post hoc analysis (treatment effect F_4,28 = 15.299, P < 0.000; time effect F_7,28 = 478.774, P < 0.000; interaction effect F_28,28 = 2.814, P < 0.000). c Lung tissue sections were prepared and subjected to H&E staining and Masson’s trichrome staining. Scale bar: 2000 μm and 100 μm (insets). d The development of lesions revealed by H&E staining (left panel, F_4,28 = 6.434, P = 0.001) and Masson’s trichrome (right panel, F_4,28 = 29.255, P < 0.000) was scored by pathologists blind to the study design. e Lung tissue collagen content was determined by hydroxyproline analysis (F_4,28 = 6.680, P = 0.001). *P < 0.05, **P < 0.01 determined by one-way ANOVA with S-N-K post hoc analysis (d, e). n = 5 (bleomycin) or n = 7 (all the other treatments) rats per group.