Fig. 1. Environmental enrichment promotes oligodendroglial maturation and myelination after perinatal brain injury.

a Schematic of the subcortical white matter (gray) in a coronal slice, with boxes representing the quantified regions (corpus callosum, cingulum, external capsule). b Experimental paradigm. c Experimental groups. d Quantification of EGFP + NG2 + OPCs at P30 (NX Standard (NX), n = 7; NX Enriched (NX-EE), n = 6; HX Standard (HX), n = 6; HX Enriched (HX-EE), n = 8) (***p = 0.0003; ****p < 0.0001). e Quantification of EGFP + Ki67 + proliferating OPCs at P30 (NX, n = 6; NX-EE, n = 5; HX, n = 7; HX-EE, n = 6) (**p = 0.002; ****p < 0.0001). f Quantification of EGFP + CC1 + OLs at P45 (n = 6 per group) (***p = 0.0001; ****p < 0.0001). g Representative western blot for myelin proteins MAG and MBP in the subcortical white matter of the four experimental groups at P45 (n = 3 per group). h Quantification of MAG protein levels, normalized to Actin (from left to right: *p = 0.038; ***p = 0.0004; *p = 0.037). i Quantification of MBP protein levels, normalized to Actin (*p = 0.03; **p = 0.009; ***p = 0.0007). j Representative EM images from P45 white matter. Experiments were performed once using CNP-EGFP mice. Scale bar = 0.5 μm. k Quantification of myelinated axon number in the corpus callosum (n = 3 per group) (*p = 0.039, **p = 0.001). l Scatter plot of calculated g-ratios of individual corpus callosum axons relative to axon diameters in HX vs. HX-EE. m Representative images of ENPP6 in situ hybridizations from P45 white matter. Experiments were performed once using CNP-EGFP mice. Scale bar = 50 μm. n Quantification of high ENPP6-expressing cells at P45 (n = 4 per group) (from left to right: ****p < 0.0001, **p = 0.003, **p = 0.008). Unless otherwise noted, data expressed as mean ± SEM. One-way ANOVA followed by a Tukey’s post hoc test for multiple comparisons was used to compare differences between groups. Source data are provided as a Source Data file.