Fig. 3. Nec-1s improved the morphological and histological features and the necroptosis-related protein expression in mice with IMQ-induced psoriasiform dermatitis.

a Schematic diagram of the animal experiment protocol for the Control, IMQ, and IMQ + Nec-1s groups (n = 6). b Representative macroscopic views and H&E staining of cross-sectional slices of the dorsal skin of BALB/c mice on the 8th day. Scale bar represents 200 μm. c The epidermal thickness of the dorsal skin on the 8th day was measured by four randomly selected fields per section of each mouse. d Daily assessment of epidermal erythema, scales, and thickening of the dorsal skin. The PASI score was calculated by adding the scores of three independent criteria (ranging from 0 to 12). e Immunohistochemical staining for RIPK1 and MLKL in the dorsal mouse skin samples for the three groups. Representative images are shown. Scale bar represents 200 μm. f Protein levels of RIPK1, pRIPK3(S232), RIPK3, MLKL and pMLKL (S345) were quantified by western blotting. GAPDH served as the loading control. Error bars represent mean ± standard deviation (SD). *p < 0.05, **p < 0.01, and ***p < 0.001 when compared. All assays were repeated three times with consistent results.