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. 2020 Feb 13;10:127. doi: 10.3389/fonc.2020.00127

Figure 2.

Figure 2

M3814 overactivates p53 in response to calicheamicin in AML cells. (A) Relative gene expression analysis of key p53 transcriptional targets, Mdm2, p21 and Puma, in MV4-11 (p53 wild-type) cells treated with DMSO, calicheamicin (0.5 or 1.0 pM), or M3814 (300 or 1,000 nM) alone or in combination. Relative expression determined by the 2(−ΔΔCt) method with GAPDH reference. (B) Western blot analysis of ATM and p53 pathway proteins as well as apoptotic indicators at 6, 24, 48, and 96 h in lysates of MV4-11 cells treated with vehicle, M3814 (1 μM), calicheamicin (1pM), or the combination of calicheamicin (1 pM), and M3814 (1 μM). (C) Relative gene expression analysis at 6 and 24 h of key p53 transcriptional targets, Mdm2, p21, and Puma, in MV4-11 (p53 wild-type) cells treated with DMSO, M3814 (1 μM), M3541 (1 μM), calicheamicin (1.0 pM), calicheamicin (1 pM) + M3814 (1 μM), or calicheamicin (1 pM) + M3814 (1 μM) + M3541 (1 μM). *P < 0.05, **P < 0.01, ***P < 0.001.