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. 2020 Feb 19;10:2932. doi: 10.1038/s41598-020-60050-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chrysin inhibits TNF-α and IL-17A-induced phosphorylation of IKK/NF-κB pathway in NHEK. The phosphorylation of IκBα protein in both TNF-α (A) and IL-17A (C) only groups was significantly upregulated under pre-treatment with chrysin, and the expression of IκBα protein was inhibited by chrysin pre-treatment. The quantification data are shown in the below panel (B,D). The total protein was extracted from the cells and IκBα expression was determined via western blotting. The results are presented as a percentage of the control. *p < 0.05 was considered to be statistically significant.