Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 19;10:2936. doi: 10.1038/s41598-020-59891-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Quantification of the doxycycline-inducible expression level of fluorescent proteins in C. albicans. (A) GFPmut2 was used as an indicator to quantify expression under the Tet-on system in a dose-dependent manner. C. albicans cells from strains WCL101, WCL102, WCL104, and WCL105 were grown in YPD with the indicated concentration of doxycycline for 3 hours, followed by western blotting to analyse the amount of GFPmut2 in the cell lysates. β-Actin was used as an internal control. Asterisks indicate non-specific signals. (B) mCherry was used as another indicator to quantify expression under the Tet-on system in a dose-dependent manner. C. albicans cells from strains WCL103 and WCL105 were grown in YPD with the indicated concentration of doxycycline for 3 hours, and their lysates were subjected to western blotting to analyse the amount of mCherry in the cells. β-Actin was used as an internal control. (C) The change in doxycycline-inducible GFPmut2 expression. The GFPmut2 and β-actin signals on the blots shown in (A) were quantified with ImageJ. The bar charts show the fold change in GFPmut2 expression normalized to the relevant β-actin expression analysed with one-way ANOVA with Bonferroni’s comparison test. (D) The change in doxycycline-induced mCherry expression. The mCherry and β-actin signals on the blots shown in (B). The bar charts were obtained with the same formula used for (C). The numbers of asterisks show the significance of the difference between two values compared by statistical analysis.