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. 2020 Feb 19;10:2936. doi: 10.1038/s41598-020-59891-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The mCherry-based BiFC system regulated by the Tet-on system in C. albicans. (A) Validation of split mCherry fragments unable to rejoin into a fluorescent complex. C. albicans cells from strains WCL103, WCL106, WCL107, WCL109, WCL110, and WCL117 were grown in YPD with and without 40 μg/ml doxycycline for 6 hours. The cells from strains WCL106, WCL107, and WCL117 increased in size. Red fluorescence in both the presence and absence of doxycycline was undetectable under fluorescent microscopy. The bar indicates 10 μm. (B) Examination of the mCherry-based BiFC system by the interaction of CaCdc42-CaRdi1. C. albicans cells from strains WCL111, WCL112, WCL113, WCL125, WCL108, and WCL118 were grown in YPD with and without 40 μg/ml doxycycline for 6 hours. Red fluorescence in the large cells of strains WCL118 and WCL108 was visualized by fluorescence microscopy. The other strain exhibited a background or undetectable signal. The bar indicates 10 μm. (C) Quantification of relative fluorescence intensity of the strains WCL111, WCL112, WCL113, WCL108, WCL125, and WCL118 with ImageJ. The values of bar charts derived from the integrated density of the original figures (Fig. S8) subtracting the background were analysed with one-way ANOVA with Bonferroni’s comparison test. ns: not statistically significant.