Table 2.
Synthetic oligonucleotides used in this study.
| Primer | Sequenceb |
|---|---|
| TDH3p SacII F | ATTACCGCGGGTTGCTCCTCGTCGACAA |
| TDH3p XbaI R | CGGGTCTAGACATTGTTAATTAATTTGATTGTAAAG |
| MCS3 F | CTAGATTTAAATATGCATTGACCGCGGATTCAAGAGTCCAAGCT |
| MCS3 R | TGGACTCTTGAATCCGCGGTCAATGCATATTTAAAT |
| MCS4 F | TGCACCAGCTCCGGTACCACTAGTCATGTTTAAACCGG |
| MCS4 R | TCGACCGGTTTAAACATGACTAGTGGTACCGGAGCTGGTGCAGTAC |
| HygB BglII F | GCTTAGATCTCCTGCAGGTATAGTGCTTGCTGTTCGATA |
| HygB NsiI R | CGTTATGCATACGCGTCATTTTATGATGGAATGAATGGG |
| TetO F | AAACTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGACATTGAGCT |
| TetO R | CAATGTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGAGTTT |
| miniOP4 SpeI F | GATTACTAGTCATTATTTATATTTGTATGTGTGTAGGAGTT |
| miniOP4 XhoI R | AGGACTCGAGCAAACTTCTTTTTTATTTTTCGCAATAT |
| TDH3t PmeI F2 | AAAAGTTTAAACACCAAACTCGAAGGTGCTC |
| TDH3t KpnI R2 | GATTGGTACCTCTGGTGGAGTAACCGTATT |
| GFPt XbaI F | TTTTCTAGACCGGTCCGACATTTTATGATGGAATGAATGG |
| GFPt SpeI R | TTTACTAGTGTCGACTCGAGATATCCAGCTA |
| mRFP NotI F | ATTTGCGGCCGCTCCGGAGTTTCAAAAGGTGA |
| mRFP AatII R | CATAGACGTCAGGCGCGCCTTTATATAATTCA |
| GS F | ATCAGATCTGGTGGAGGTG |
| GS R | GATCCACCTCCACCAGATCTGAT |
| mRFP N BamHI F | AACTGGATCCATGGTTTCAAAAGGTGAAGAAGATAATATGGC |
| mRFP N NotI R | TCTAGCGGCCGCAGGCCTTTAATCTTCTGGATACATTCTTTCTGATGAAGC |
| mRFP C XhoI F | TCAACTCGAGATGGGTGCTTTAAAAGGTGAAATTAAACAAAGA |
| mRFP C BamHI R | TACTGGATCCTTTATATAATTCATCCATACCACCAGTTGA |
| CaGFP-N-MCS | AATTGTCGACTCGAGATATCCAGCTAGCGGCCGCGTGACCATGAGTAAGGGAGAAGAAC |
| CaGFP-C-MCS | CCGTGATCATTATGCAGGCCTAGATCTTAAGCTGACGTCCGGACCTTTGTATAGTTCATCCATGCC |
| CDC42 EcoRV F | CCTAGATATCCAAACTATAAAATGTGTTGTTGTCGGTG |
| CDC42 BglII R | GGGTAGATCTTAAAATAGTACACTTTTTCGATTTTTTAAT |
| RDI1 SpeI F | GATTACTAGTTCCAATTCTAACGTTGATGATGAT |
| RDI1 StuI R | GCTTAGGCCTCTATTTAGTAATGGAAAAACTCCAAGG |
| RedN KpnI F | CCAAGGTACCACTAGTATGGTTTCAAAAGGTGAAGAAGATAATATGGC |
| RedN EcoRV | CTTTGATATCATCTTCTGGATACATTCTTTCTGATGAAGC |
| CaCDC42 BamHI F | ATAAGGATCCGACGTCATGCAAACTATAAAATGTGTTGTTGTCG |
| CaCDC42 NotI R | ATAAGCGGCCGCAGGCCTCTATAAAATAGTACACTTTTTCGATTTTTTAAT |
| pNIM1 inte F | CATGTCAAAGGATTCAAC |
| pNIM1 inte R | GTATGGTGCCTATCTAAC |
| TDH3 F | TTTGGTTGCGTTAGTCCG |
bRestriction enzyme sites included in primers are highlighted in bold italics.