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. 2020 Feb 19;10:2974. doi: 10.1038/s41598-020-59900-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection limit of the duplex PCRs for both Ophiocordyceps sinensis and its host Hepialiae species. The template was genomic DNA extracted from reference material (No. 121201) and subjected to a fivefold serial dilution, resulting in approximately 20,000, 4,000, 800, 160, 32, 6.4, 1.3, 0.25 and 0.05 pg per PCR reaction. The DL2000 DNA Marker was used as the size scale for the target DNA. Sterile water was used as the negative blank control. The simultaneous appearance of the two target bands was considered a positive result and marked “+”. Others marked “−” were negative.