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. 2020 Feb 1;23(2):100878. doi: 10.1016/j.isci.2020.100878

Figure 1.

Figure 1

Evaluation of miR-34a Expression and Dependence in a Panel of Lung Cancer Cell Lines

(A and B) (A) Distribution of miR-34a expression in non-small cell lung cancer cell lines compared with normal lung epithelial cells SALE and AALE. Compared with SALE, all cancer cell lines showed a significant reduction in miR-34a levels (p < 0.001), and compared with AALE, except for H650 and H358, all the cancer cell lines showed a significant reduction in miR-34a levels (p < 0.001). (B) miR-Control and miR-34a dose-response curves in non-small cell lung cancer cell lines (n = 10). Cells were transfected with miR-Control or miR-34a for 72 h, and viability was measured using the MTT reagent.

(C) Data showing qPCR-based measurements of miR-34a downstream genes BCL2, CD44, JAG1, MET, and MYC, after treating A549 cells with miR-Control and miR-34a mimics at 40 nM for 72 h. All comparisons are done with respective miR-Control gene expression (*p < 0.05, **p < 0.005, Student's t test). Bars and errors bars represent the means and the corresponding SEMs for n ≥ 3.