Figure 5.
c-Rel Recruits the Co-repressor HDAC1 to the RelA-Dependent Promoters
(A) Wild-type or c-Rel knockout MEFs (30 × 106 cells/condition at time of harvest) were left untreated or treated in 3 × 15-cm plates per condition with 100 ng/mL TNF-α for 15 min. Chromatin immunoprecipitation (ChIP) was performed using control IgG or RelA antibody.
(B) Wild-type MEFs were treated as in (A) and ChIP was performed using anti-c-Rel or anti-RelA antibodies. Enrichment of IP-10 and CXCL1 promoter regions in the ChIP samples were examined by qPCR in triplicates.
(C). Wild-type MEFs were treated with TNF-α for 15 min, and nuclear lysates were immunoprecipitated using anti-c-Rel antibody and examined for binding to the indicated HDACs.
(D). Oligonucleotide pull-down assay was performed as in Figure 4D, and the precipitated proteins were separated in SDS/PAGE gel and probed for indicated HDACs and c-Rel.
(E) Wild-type and c-Rel KO cells were treated as in (A), and ChIP was performed using anti-HDAC1 antibody.
(A)–(E) n = 4. Data are presented as mean ± standard error of mean (SEM). p Values were obtained by unpaired Student’s t test; ***p < 0.001, **p < 0.01, *p < 0.05.