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. 2020 Feb 19;10:2919. doi: 10.1038/s41598-020-59719-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of SAD L16 GFP in the t. palatina of different reservoir species by RT-qPCR. All animals received 10^8.0 FFU/mL SAD L16 GFP by direct oral instillation. SAD L16 GFP positive (green dots): Ct values < 38, SAD L16 negative: Ct values ≥ 38 (dotted line, values not shown). t. palatina, which were tested positive for RABV RNA in RT-qPCR screenings, were tested for infectious virus by RTCIT. Samples for which infectious vaccine virus could be isolated are highlighted with a red circle.