Figure 3.
MiR-20a-5p was down-regulated and associated with inflammation during liver fibrosis. (A) miR-20a-5p expression in the clinical samples from normal (n = 10) or liver fibrosis patients (n = 20) were analyzed by qRT-PCR. (B) The schematic of the in-vitro cell model. (C) The concentration-response curves of CCl4 for hepatocyte injury model. (D) The cytokine levels of IL6, TNF-α, and IL-18 were determined in control cells and CCl4-cells transfected with miR-20a-5p or their respective NCs by ELISA and qRT-PCR. *p < 0.05, **p < 0.01, ***p < 0.001, ###p < 0.001, respectively. *Compared with CCl4 plus miR-NC and #compared with the control.