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. 2020 Feb 13;11:33. doi: 10.3389/fneur.2020.00033

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Copyright © 2020 Damasceno, Gómez-Nieto, Garcia-Cairasco, Herrero-Turrión, Marín and Lopéz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolabeling of NPY, RGS2, and TTR proteins in the inferior colliculus (IC) of the control Syrian hamster and the GASH/Sal model at naïve and sound-stimulated conditions. The inset in (A) depicts a representative schematic section of the IC (6.00 mm caudal from bregma), in which the immunoreactivity was analyzed. For each protein, upper panels show lower-magnification photomicrographs of the IC and lower panels show higher magnifications corresponding to the frame in the upper panels. (A) Weak NPY-immunopositive perikarya (arrows) are shown in GASH/Sal animals at naïve and sound-stimulated conditions, whereas naïve control Syrian hamster exhibit absence of NPY-immunolabeling (asterisks). (B) Moderate nuclear immunolabeling for RGS2 protein (arrows) is found in GASH/Sal animals at naïve and sound-stimulated conditions. Notice the absence of RGS2-immunolabeling (asterisks) in the control animal. (C) An increase in immunolabeling of TTR (arrows) is shown in GASH/Sal animals at naïve and sound-stimulated conditions when compared to naïve Syrian hamsters (asterisks). Notice the distribution of TTR-immunolabeling outlining the cell bodies of unlabeled IC neurons (arrows). Scale bars for upper panels and lower panels are 100 and 20 μm, respectively.