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. 2020 Feb 19;10:2986. doi: 10.1038/s41598-020-59818-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The immunoreactivity of the fully human antibody GB235 against HER2. (A) GB235 specifically bound to soluble human HER2 ECD but not to the other human EGFR family receptors. (B) Flow cytometry analysis of GB235 binding to HER2-overexpressing BT-474 cells. MFI: mean fluorescence intensity. GB235 was evaluated for its activity to compete with biotinylated Trastuzumab (C) and biotinylated Pertuzumab. (D) The results are representative of three different experiments and expressed as the mean ± SEM.