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. 2020 Feb 19;10:2986. doi: 10.1038/s41598-020-59818-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of GB235 alone or in combination treatment with Trastuzumab in NCI-N87 cells. (A) Enhanced proliferation inhibition with combination treatment of GB235 and Trastuzumab was shown in NCI-N87 cells in the presence of heregulin-α. (B) Western blot analysis of HER3 and downstream signaling pathway activation upon GB235 (20 µg/mL) or Trastuzumab (20 µg/mL) alone, or the two antibodies in combination treatment followed by heregulin-α stimulation.