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. 2020 Feb 19;10:2957. doi: 10.1038/s41598-020-59899-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activated Wnt signaling through stabilized β-catenin does not alter Wnt-mediated signaling pathway component localization. Representative images of HCT116βm cells (CRC line with single allele encoding β-catenin that cannot be phosphorylated by CK1-α) treated with UB or WB as in Fig. 1a. Phosphorylation by CK1-α is reported to be a prerequisite for phosphorylation by GSK-3β and subsequent ubiquitin-mediated degradation. Representative images and scoring shown for (a) Axin1, (b) β-catenin, (c) CK1-α, (d) GSK-3β, (e) Frizzled-7, and (f) β-TrCP. Data averaged from three independent experiments (n = 25 cells per condition). Error bars, SEM; Statistical analysis by t-test: *P < 0.05; **P < 0.01; ***P < 0.001. Representative line scans provided in supplemental Fig. 2. Scale bar, 10 μm.