Skip to main content
. 2020 Feb 19;11:956. doi: 10.1038/s41467-020-14750-x

Fig. 5. Comparison of the corepressor and coactivator interaction network.

Fig. 5

a Interactions with six residues in the PPARγ AF-2 surface mediate binding to the helical core of the NCoR ID2 peptide (PDB 6ONI). b Fewer interactions mediate binding to the helical core of the TRAP220 coactivator peptide (PDB 6ONJ). c NCoR ID2 peptide affinities and errors (mean ± s.d. of the fit to a one site—total binding equation) from fluorescence polarization peptide interaction assays using structure-guided mutants (Supplementary Fig. 4) displayed to illustrate the difference in NCoR ID2 peptide binding affinity between apo- and T0070907-bound PPARγ LBD. d Cell-based luciferase reporter assay in HEK293T cells treated with DMSO control or 5 μM T0070907 measuring the ligand-dependent change in wild-type or mutant PPARγ transcription; data normalized to each WT or mutant construct vehicle control (DMSO) condition (n = 4; mean ± s.e.m.). Source data are provided as a Source Data file.