Fig. 9. T0070907 adopts different activity-dependent binding poses.

a Structural overlay highlighting the T0070907 binding pose in the crystal structure of PPARγ LBD bound to T0070907 and NCoR ID2 peptide (orange; PDB 6ONI) compared with the crystal structure of T0070907-bound PPARγ LBD without a coregulator peptide (blue; PDB 6C1I). b, c 2D [¹H,¹⁵N]-TROSY-HSQC spectra from the PRE analysis of MTSL-labeled T0070907-bound [K474C]-PPARγ LBD without coregulator peptide. Purple boxes in (b) highlight residues shown the zoomed-in snapshots (c) showing that only one of two slowly exchanging populations corresponding to the active (labeled “a”) and repressive (labeled “r”) is affected by the MTSL spin label on helix 12.