Fig. 1.
a Structure of ellagic acid. Molecular formula C14H6O8. Molecular weight 302.19 g/mol. Effect of ellagic acid on various physiological parameters in flies. b Effect of ellagic acid on body weight of wild-type Canton-S flies. Data represented as body weight (mg/10 flies). Data were subjected to two-way ANOVA followed by Bonferroni’s multiple comparisons test in the GraphPad Prism software v.6. Error bars denote standard deviation. Symbols represented as *p < 0.05 and ****p < 0.0001. Twenty vials containing 10 flies/vial were counted for each control, and treatment with EA 100 μM and EA 200 μM. The total number of flies used was 1200. c Effects of ellagic acid on fly longevity. Ten vials containing 30 flies/vial were monitored for lifespan. The total number of flies used was 1800. Mean lifespan was calculated by the Kaplan–Meier lifespan analysis in the OASIS2 software. Mean values of individual replicate of the control, and two treatments of male and female flies were fed into the GraphPad Prism v.6 software. Data were subjected to two-way ANOVA followed by Bonferroni’s multiple comparisons test. Data represented as mean lifespan (in days) with error bars denoting SD. Symbols represented as *p < 0.05 and ****p < 0.0001. d Effect of ellagic acid on female fecundity. For each time point and each treatment, eggs from ten vials containing 1 male and 1 female flies/vial were counted to get the value for row means with SD. The mean number of eggs laid per female per day was used to get the fecundity curve. Data were subjected to one-way ANOVA followed by Holm–Sidak’s multiple comparisons test in the GraphPad Prism software v.6. Significance p < 0.005. e Effect of ellagic acid on climbing efficiency in flies. Data represented as mean time (in seconds) taken by 10 flies per replicate per treatment to climb 15 cm, with error bars denoting SD. For each treatment and sex along with their respective control, twenty vials containing 10 flies/vial were counted. The total number of flies used was 1200. Data were subjected to two-way ANOVA followed by Bonferroni’s multiple comparisons test in GraphPad Prism software v.6