Fig. 3.

D3-5 feeding improves aging-related ailments like physical function, muscle strength, depression/anxiety, learning-memory, leaky gut, and inflammation in older obese mice. a, b Physical function [measured as ambulatory activity (a) and total activity (b)], muscle strength [measured as inclining on tilted screen (c)], anxiety/depression behavior [measured as rearing (d) and time spent in center during open field test (e–g)], and learning-memory behavior [measured during Morris Water Maze test (h)] were significantly enhanced in D3-5 fed older obese mice compared to their controls. Leaky gut [measured by appearance of FITC in blood leaked from gut (i)] and mRNA expression of inflammatory markers (like IL-6 and IL-1β) were significantly decreased in D3-5 fed older obese mice compared to their age-sex-matched controls (j, k). Values are mean of n = 5–8 mice per group ± SEM (error bars). Values with *p < 0.05, **p < 0.001 are statistically significant. ns: not significant