Figure 1.

Biofilm preparation and treatment regimen. The saliva inoculum was inoculated in 1.5 ml of SHI medium and cultured without disturbance during the first 24 h to form an initial biofilm community on the enamel block surface. Starting at 72 h, the biofilms were transferred to SHI medium containing different dietary sugars (i.e., 2% sucrose, 2% lactose and 2% glucose) to simulate cariogenic challenges until the endpoint (144 h). The culture medium was changed every 24 h until the end of the experimental period. The control group was saliva derived biofilm cultured with SHI medium only.