Figure 3.

PMT12-BF4 inhibits C. albicans from germination. (A) C. albicans SC5314 overnight culture was diluted with 1 mL RPMI 1640 medium to OD₆₀₀ 0.25, and PMT12-BF4 was added at the indicated concentrations. After incubation for 3 h at 37 °C, cells were observed with an optical microscope at 400×. Scale bar = 20 μm. (B) C. albicans overnight culture was diluted with 3 mL RPMI 1640 medium to 10⁵ CFU/mL, and PMT12-BF4 was added at the indicated concentrations. After incubation for 3 h at 37 °C, cells were pre-fixed with 2% glutaraldehyde, washed with PBS, pro-fixed with 1% OsO₄, and dehydrated with a series of ethanol washes. Cells were dried with a critical point dryer. Gold was coated on samples before scanning electron microscopic observation. Scale bar = 5 μm.