Figure 2.

Sanger sequencing confirmation for two CSF3R mutations (A). TA cloning to identify whether the two CSF3R mutations were in the same DNA strand or not (B). In the lanes of 1, 3, 5, 6, and 10, c.1853C>T and c.2337T>G were found simultaneously, while no CSF3R mutations were found in other lanes. These findings suggest that the two CSF3R mutations are located in the same DNA strand, finally indicating that the two mutations are harbored in the same leukemic clone