Table 1.
Method validation data: retention time (Rt) and analytes concentration for Cal and QC used for method validation are written in bold. Mean back‐calculated concentration according to regression equations (n = 6) are shown in italic
| Analyte | Rt (min) | Cal 1 [µmol/L] | Cal 2 [µmol/L] | Cal 3 [µmol/L] | Cal 4 [µmol/L] | Cal 5 [µmol/L] | Cal 6 [µmol/L] | QC high [µmol/L] | QC Med [µmol/L] | QC low [µmol/L] | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Bias (%) | RSDT (%) | RSDR (%) | Bias (%) | RSDT (%) | RSDR (%) | RE (RSD) | ( %) | Plasma | RE (RSD) | (%) | Urine | Bias (%) | RSDT (%) | RSDR (%) | ||||||||
| DMA | 2.3 | 12 500 | 8000 | 4000 | 400 | 50 | 25 | 10 000 | 1000 | 75.4 (5.1) | 112.5 (5.2) | 33.3 | ||||||||||
| 12 093.5 | 8202.6 | 4370.5 | 816.8 | 137.1 | 23.1 | −4.0 | 5.3 | 5.0 | −1.5 | 2.3 | 3.8 | −6.6 | 11.7 | 9.6 | ||||||||
| TMA | 2.56 | 500 | 320 | 160 | 16 | 2.0 | 1.0 | 400 | 40 | 92.6 (7.0) | 93.0 (3.4) | 1.3 | ||||||||||
| 498.2 | 319.0 | 163.3 | 32.5 | 6.2 | 0.9 | 0.2 | 6.8 | 6.0 | −1.6 | 1.2 | 6.8 | −3.6 | 2.1 | 4.9 | ||||||||
| IPA (IS) | 3.27 | |||||||||||||||||||||
| DEA | 5.47 | 5000 | 3200 | 1600 | 160 | 20 | (10) | 4000 | 400 | 97.2 (4.5) | 99.0 (3.4) | 13.3 | ||||||||||
| 4806.4 | 3321.7 | 1766.0 | 329.6 | 51.4 | 9.3 | −6.8 | 6.4 | 5.4 | −6.9 | 1.8 | 6.0 | −11.5 | 12.6 | 12.6 | ||||||||
| TEA | 7.35 | 250 | 160 | 80 | 8.0 | 1.0 | 0.5 | 200 | 20 | 98.1 (6.4) | 104 (3.6) | 0.67 | ||||||||||
| 243.1 | 163.7 | 86.2 | 16.3 | 2.8 | 0.5 | 3.8 | 6.0 | 5.9 | 5.9 | 2.2 | 3.7 | −3.2 | 5.9 | 8.4 | ||||||||
Bias, intra‐day precision (RSDR), inter‐day precision (RSDT), and recovery efficiency (RE) are given in percent (%).