Figure 1.

PCR array analyses of osteogenic genes. The gene expression was evaluated in human adipose mesenchymal stem cells grown on Coll/Pro Osteon200 compared with tissue culture polystyrene. A, Induced genes at day 21. In hASC cultures, mRNAs of 22 genes of the osteogenic pathway, that is, ALPL BGLAP BMP1/2, BMPR1B, COL1A1,CSF2/3, EGFR, FGFR1, FGFR2, IGF1, IGF1R, NOG,RUNX2, SOX9, SP7, SPP1, TGFB1, TNFSF1, TWIST1, and VDR were upregulated; one gene, such as VCAM1 was downregulated. B, Induced genes at day 40. In hASC culture, mRNAs of 10 genes of the osteogenic pathway, that is, BMP2, BMPR1B, CSF2, CSF3, EGF, EGFR, ITGA2, SOX9, SPP1, and TNFSF11 were upregulated, whereas nine genes were downregulated (BMP6, CD36, COMP FGFR2, IGF2, ITGAM, NOG TGFB3, and VCAM1)