Fig. 4.

SDS-PAGE of SGE proteins and IgE Immunoblots of mosquito-allergic patient sera to SGE of the four mosquito species. SDS-PAGE of SGE proteins (left), as well as IgE immunoblots (right) from 10 representative patient samples for each of the indicated mosquito species, are shown. Arrows on IgE immunoblots indicate major IgE reactive bands. An arrow on each SDS-PAGE indicates the corresponding protein band cut and subjected to mass spectrometry for the identification of novel allergen