Figure 5.

Induction of senescence plays a role in IGFBP7 reprograming of fibroblasts to osteoblasts. Fibroblasts were treated without or with IGFBP7 (1000 ng/mL) and gene expression was measured by qPCR. IGFBP7 significantly increased expression of key senescence‐associated genes (P16, P21, and P53; A‐C) and SASP‐associated genes (IL‐1α, TNF‐α, and IL‐6; D‐F) at day 4 or day 14. *P ≤ .05 when comparing to control at day 14 using a Student's t test. Data were replicated in four independent experiments