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. 2020 Feb 13;11:65. doi: 10.3389/fpls.2020.00065

Figure 3.

Figure 3

Expression of P. viticola NLPs 1 to 3 during infection of V. vinifera cv. Mueller-Thurgau: (A) Fluorescence microscopy pictures of Aniline Blue stained leaf disks representing different P. viticola stages during the infection of V. vinifera cv. Meuller-Thurgau: i: 1 hpi, ii: 3 hpi, iii: 6 hpi, iv: 24 hpi; gt, germ tube; h, haustoria; hy, hypha; sp, sporangium; st, stoma; zs, zoospore; scale bars = 20µM. (B) Relative expression of PvNLP1, PvNLP2, and PvNLP3 on infected V. vinifera cv. Mueller-Thurgau leaf disks at the time points: zoospore release (1 h), penetration (3 h), first haustoria visible (6 h), branching of hyphae (24 h), 72h, 96h, and 144 h after inoculation. The displayed graph represents one of three experiments with similar results. Bars are the mean of three technical replicates ± SD. (C) Relative expression of PvNLP1, PvNLP2, and PvNLP3 in a sporangia solution with (+) or without (−) presence of leaf material at the time points 15, 30, 60 (beginning of zoospore release from the sporangia), and 150 min after water contact. The displayed graph represents one of three experiments with similar results. Bars are the mean of three technical replicates ± SD. Asterisks indicate significance (p ≤ 0.01, one-way Anova).