HPLC analysis and LC-UV chromatogram of GBHT. The HPLC analysis was performed in a reverse-phase column C18, gradient mobile system of acetonitrile in 0.001% phosphoric acid, flow rate set at 1.0 mL/min, and an LC-UV chromatogram of GBHT was recorded at UV 203 nm (A, upper panel), UV 254 nm (B, upper panel), and UV 276 nm (C, upper panel). Representative LC-UV chromatograms of standards of mangiferin, ginsenoside Rg1, ginsenoside Rg3, glycyrrhizic acid, liquiritin apioside, and liquiritin that were recorded at UV 203 nm (A, lower panel), UV 254 nm (B, lower panel), and UV 276 nm (C, lower panel).
GBHT, ginseng-plus-Bai-Hu-Tang; GA, glycyrrhizic acid; HPLC, high performance liquid chromatography; LA, liquiritin apioside; L, liquiritin; M, mangiferin; Rg1, ginsenoside Rg1; Rg3, ginsenoside Rg3.