. 2019 Oct 9;117(10):1963–1980. doi: 10.1016/j.bpj.2019.10.003

Table 3.

Initial Concentrations and ODEs

Species	Concentration (nM)	ODE
Ca²⁺	100	$(d [C a^{2 +}] / d t) = - 2 R_{1} - 2 R_{2} - 2 R_{4} - 2 R_{6}$
CaM	1240	$d [C a M] / d t = - R_{1}$
Ca₂ ⋅ CaM	0	$(d [C a_{2} C a M] / d t) = R_{1} - R_{2} - R_{3} - R_{5}$
Ca₄ ⋅ CaM	0	$(d [C a_{4} C a M] / d t) = R_{2} - R_{26}$
AC1	5000	$(d [A C 1] / d t) = - R_{3} - R_{25}$
AC1 ⋅ Ca₂ ⋅ CaM	0	$(d [A C 1 C a_{2} C a M] / d t) = R_{3} - R_{4}$
AC1 ⋅ Ca₄ ⋅ CaM	0	$(d [A C 1 C a_{4} C a M] / d t) = R_{4}$
PDE1	3800	$(d [P D E 1] / d t) = - R_{5} - R_{11}$
PDE1 ⋅ Ca₂ ⋅ CaM	0	$(d [P D E 1 C a_{2} C a M] / d t) = R_{5} - R_{6}$
PDE1 ⋅ Ca₄ ⋅ CaM	0	$(d [P D E 1 C a_{4} C a M] / d t) = R_{6}$
ATP	$2 \times 10^{6}$	$(d [A T P] / d t) = - R_{7} - R_{27}$
cAMP	0	$(d [c A M P] / d t) = R_{7} - 2 R_{8} - 2 R_{9} - R_{13} - R_{14} - R_{15} + R_{27}$
R₂C₂	220	$(d [R_{2} C_{2}] / d t) = - R_{8}$
R₂C₂ ⋅ cAMP₂	0	$(d [R_{2} C_{2} c A M P_{2}] / d t) = R_{8} - R_{9}$
R₂C₂ ⋅ cAMP₄	0	$(d [R_{2} C_{2} c A M P_{4}] / d t) = R_{9} - R_{10}$
R₂ ⋅ cAMP₄	0	$(d [R_{2} c A M P_{4}] / d t) = R_{10}$
PKAc	10	$(d [P K A c] / d t) = 2 R_{10}$
PDE1P	0	$(d [P D E 1 P] / d t) = R_{11}$
PDE4	1000	$(d [P D E 4] / d t) = - R_{12}$
PDE4P	0	$(d [P D E 4 P] / d t) = R_{12}$
AMP	0	$(d [A M P] / d t) = R_{13} + R_{14} + R_{15}$
ISO	4^a	$(d [I S O] / d t) = - R_{16} - R_{20}$
β₂AR	19.2^a	$(d [β_{2} A R] / d t) = - R_{16} - R_{19}$
ISO ⋅ β₂AR	1.2^a	$(d [I S O \cdot β_{2} A R] / d t) = R_{16} - R_{17} + R_{22}$
G_sαβγ	3654^a	$(d [G_{s α β γ}] / d t) = - R_{17} - R_{19} + R_{24}$
ISO ⋅ β₂AR ⋅ G_sαβγ	1.2^a	$(d [I S O \cdot β_{2} A R \cdot G_{s α β γ}] / d t) = R_{17} - R_{18} + R_{20} - R_{21}$
ISO ⋅ β₂AR ⋅ G_βγ	0	$(d [I S O \cdot β_{2} A R \cdot G_{β γ}] / d t) = R_{18} + R_{21} - R_{22}$
β₂AR ⋅ G_sαβγ	709.2^a	$(d [β_{2} A R \cdot G_{β γ}] / d t) = R_{19} - R_{20}$
G_sαGTP	1.2^a	$(d [G_{s α} G T P] / d t) = R_{18} + R_{21} - R_{23} - R_{25}$
G_sαGDP	0	$(d [G_{s α} G D P] / d t) = R_{23} - R_{24}$
G_βγ	118.8^a	$(d [G_{β γ}] / d t) = R_{22} - R_{24}$
AC1 ⋅ G_sαGTP	52.8^a	$(d [A C 1 \cdot G_{s α} G T P] / d t) = R_{25} - R_{26}$
AC1 ⋅ G_sαGTP ⋅ Ca₄ ⋅ CaM	1.2^a	$(d [A C 1 \cdot G_{s α} G T P \cdot C a_{4} C a M] / d t) = R_{26}$

All the initial concentrations are estimated in COPASI using the experimental data fitted to the model parameters. Membrane-bound molecule concentrations are converted from $(mol / μ m^{2})$ to nM by volume to surface ratio of the compartment.

The concentrations are taken from (43).