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. 2019 Aug 13;44(2):341–349. doi: 10.1016/j.jgr.2019.08.002

Fig. 1.

Fig. 1

Senescence-β-galactosidase activity assay of Rg3(S)-treated senescent HDFs. (A) Light microscopic images of young HDFs (passage #10) and old HDFs (passage #35) pretreated with 10 μM and 30 μM Rg3(S) for 48 h at 37°C. (B) Quantification of SA-β-gal-% positive cells of young and Rg3(S)-treated old HDFs. The percentages of SA-β gal-positive cells were calculated by SABIA (eBiogen, Seoul, Korea) using optical microscopy images. Data represent the mean ± SD of three independent experiments. ***Statistical significance p < 0.001 . HDF, human dermal fibroblast; SA-β-gal, senescence-associated β-galactosidase; SD, standard deviation.