Fig. 3.

Levels of ROS, ATP, cAMP in Rg3(S)-treated senescent HDFs. (A) ROS, (B) ATP, (C) cAMP levels were measured in young and Rg3(S)-treated old HDFs as described in Materials and Methods. Cells were stained with dichlorofluorescein diacetate, fixed, and immediately analyzed using a multi-analytic validation system (SpectraMax M4). The data represent the mean ± SD of three independent experiments. *Statistical significance p < 0.05, ** p < 0.01, NS : No Significance ROS, reactive oxygen species; SD, standard deviation; HDF, human dermal fibroblast.