Figure 1.

Spheroids mimic the embryonic stages of tissue development, optimizing the multilineage differentiation capacity of Adipose stromal/stem cells and mesenchymal stromal/stem cells. A: Adipose stromal/stem cells (ASCs) and mesenchymal stromal/stem cells (MSCs) can be harvested from monolayer and seeded into 3D culture plate dishes; B: The cell suspension starts to establish cell-cell interaction in a process known as self-assembly; C: The resulting spheroids containing cells and extracellular matrix components, are described for optimizing the stemness; D: Multilineage differentiation capacity of ASCs and MSCs under appropriate morphogens.