Figure 1.
Growth (top panels) and final substrate concentration (bottom panels) of the reference labile substrate (0.1% w/v pyruvate and 0.1% v/v glycerol, respectively, as tested in Supporting Information Figure S3) and three different plasticizers (0.1% v/v), as well as phthalate (0.02% w/v) by Mycobacterium sp. DBP42 and Halomonas sp. ATBC28. Points and error bars show means and standard deviations, respectively, of three (top) and two (bottom) biological replicates. Substrate consumption graphs also show controls with either no bacterial inoculum (solid black bars) or no plasticizer (“-“; colored bars with hatched lines). ND denotes that the compound was below the limit of detection. Growth of Mycobacterium sp. DBP42 and Halomonas sp. ATBC28 on three additional plasticizers, DINP, DIDP, and TOTM, is shown in Supporting Information Figure S5.